中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (51): 9551-9557.doi: 10.3969/j.issn.2095-4344.2012.51.010

• 组织工程口腔材料 tissue-engineered oral materials • 上一篇    下一篇

四种根管封闭剂的体外抑菌性能

陈耀忠1，陈建明2，刘根娣1   

  • 出版日期:2012-12-16 发布日期:2012-12-16

Antimicrobial activity of four kinds of root canal sealers in vitro

Chen Yao-zhong1, Chen Jian-ming2, Liu Gen-di1   

  • Online:2012-12-16 Published:2012-12-16

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摘要：

背景：根管封闭剂的抑菌性能有助于去除残留于根管内的微生物，是影响根管治疗预后的一项重要因素。
目的：检测AH plus、Vitapex、Guttaflow及Endomethasone等4种根管封闭剂的体外抑菌性能。
方法：采用琼脂扩散实验测定AH plus、Vitapex、Guttaflow及Endomethasone 等4种根管封闭剂、盐酸米洛环素或咪康唑霜剂(阳性对照)组及生理盐水(阴性对照)组对粪肠球菌、金黄色葡萄球菌、大肠杆菌及白色念珠菌等微生物的体外抑制作用，24 h后检测各组材料抑菌环的大小。
结果与结论：Endomethasone根管封闭剂对上述4种微生物均有良好的抑制作用，但其对金黄色葡萄球菌、大肠杆菌及白色念珠菌的抑制作用显著低于阳性对照组(P < 0.01)，对粪肠球菌的抑制作用强于阳性对照组(P < 0.01)。    AH plus根管封闭剂仅对白色念珠菌有抑制作用，但抑制作用显著小于Endomethasone根管封闭剂及阳性对照组  (P < 0.01)。Vitapex及Guttaflow根管封闭剂对上述4种微生物无抑制作用；表明Endomethasone根管封闭剂的体外抑菌作用最强，AH plus根管封闭剂次之，而Vitapex及Guttaflow根管封闭剂无体外抑菌作用。

Abstract:

BACKGROUND: Antimicrobial activity of root canal sealers is an important factor in the prognosis of root canal treatment, which can be helpful in destroying any remaining root canal microbes.
OBJECTIVE: To determine in vitro antimicrobial activity of four kinds of root canal sealers (AH plus, Vitapex, Guttaflow and Endomethasone).
METHODS: The antimicrobial effects of AH plus, Vitapex, Guttaflow, Endomethasone, the minocycline hydrochloride or miconazole nitrate cream (positive control group) and normal saline (negative control group) against the following microbes: Enterococcus faecalis, Staphylococcus aureus, Escherichia coli and Candida albicans were evaluated using the agar diffusion test in vitro. The diameters of the zones of microbial growth inhibition were measured after 24 hours.
RESULTS AND CONCLUSION: Endomethasone demonstrated an antimicrobial activity for all tested microbes. The mean antimicrobial efficacy of Endomethasone against Staphylococcus aureus, Escherichia coli and Candida albicans was significantly less as compared with that of the positive control group (P < 0.01); however, the inhibition effect on Enterococcus faecalis was more effective than that on the positive control group (P < 0.01). For AH plus, only Candida albicans was inhibited and presented smaller mean zone of microbial growth inhibition than Endomethasone and the positive control group (P < 0.01). No antimicrobial activity was detected for Vitapex and Guttaflow. It indicats that antimicrobial activity of the four kinds of root canal sealers is greatest for Endomethasone, followed by AH-Plus, whilst Vitapex and Guttaflow have no antimicrobial activity.